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main:faqandtips [2018/02/02 15:47] bshirley |
main:faqandtips [2018/06/26 19:26] (current) bshirley |
In almost all circumstances, it is preferable to use the [[main:curvecalibrationwizard | calibration curve wizard]] when generating a [[main:calibrationcurve | calibration curve]] and the [[main:doseestimationwizard | dose estimation wizard]] to perform [[main:estimatedose | dose estimation]]. We recommend exclusively using the wizards when using ADCI for the first time, then after you have become more familiar with ADCI functionality to manually enter values when/if specific functionality is required which the wizard cannot provide. Using wizards remove several potential sources of error such as the application of the appropriate [[main:imageselectionmodel | image selection model]] during dose estimation. | In almost all circumstances, it is preferable to use the [[main:curvecalibrationwizard | calibration curve wizard]] when generating a [[main:calibrationcurve | calibration curve]] and the [[main:doseestimationwizard | dose estimation wizard]] to perform [[main:estimatedose | dose estimation]]. We recommend exclusively using the wizards when using ADCI for the first time, then after you have become more familiar with ADCI functionality to manually enter values when/if specific functionality is required which the wizard cannot provide. Using wizards remove several potential sources of error such as the application of the appropriate [[main:imageselectionmodel | image selection model]] during dose estimation. |
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| === Does ADCI require TIF images === |
| TIF images are preferred. We have also worked with jpeg images, however they exhibit loss of resolution due to their compression algorithm. The [[main:processtime | ADCI sample process time]] page shows the difference in resolution and speed of processing. The jpeg images are labelled 912x732 pixels in this page. Supported image formats are also discussed on the [[main:sample | sample]] page under the heading "Supported image formats". |
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| === How many images should I prepare for each dose point === |
| The most important thing about the images is that they should contain exclusively or primarily metaphase cells. ADCI will process images that don't have metaphases, but these reduce the speed of processing and image selection, and can affect the image selection criteria. Typically, more images (several thousand) are required at low dose than at high dose (500-1000). The system provides a warning about sample quality if there are fewer than 100 dicentric chromosomes detected in a sample (these are the criteria in the IAEA manual). This means that for calibration samples, 2000-3000 images will provide good confidence intervals. But it all depends on the quality of your data. If many images contain a lot of sister chromatid separation, then ADCI needs more images to find those images that show strong cohesion between chromatids. |
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| === Can I continue to use curves/samples generated using an older version of ADCI after upgrading to a new version === |
| It is important to note dicentric detection may vary across ADCI versions. Reasons for this variation include dicentric detection algorithm upgrades and software bug fixes. It is therefore recommended to analyse both calibration and test samples using the same version of ADCI. |
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===== Useful tips and tricks ===== | ===== Useful tips and tricks ===== |