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main:faqandtips [2018/02/07 14:25]
bshirley
main:faqandtips [2018/06/26 19:26]
bshirley
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 === How many images should I prepare for each dose point === === How many images should I prepare for each dose point ===
 ​The most important thing about the images ​i​s that they should contain exclusively or primarily metaphase cells. ADCI will process images that don't have metaphases, but the​se​ reduce the speed of processing and image selection​,​ and can affect the image selection criteria​. Typically​,​​ more images ​(several thousand) ​are required at low dose than ​at ​high dose​ (500-1000)​. The system provides a warning about sample quality if there are fewer than 100 dicentric chromosomes detected in a sample (these are the criteria in the IAEA manual). This means that for calibration samples, 2000-3000 images will provide good confidence intervals. But it all depends on the quality of your data. If many images contain a lot of sister chromatid separation, then ADCI needs more images to find those images that show strong cohesion between chromatids. ​ ​The most important thing about the images ​i​s that they should contain exclusively or primarily metaphase cells. ADCI will process images that don't have metaphases, but the​se​ reduce the speed of processing and image selection​,​ and can affect the image selection criteria​. Typically​,​​ more images ​(several thousand) ​are required at low dose than ​at ​high dose​ (500-1000)​. The system provides a warning about sample quality if there are fewer than 100 dicentric chromosomes detected in a sample (these are the criteria in the IAEA manual). This means that for calibration samples, 2000-3000 images will provide good confidence intervals. But it all depends on the quality of your data. If many images contain a lot of sister chromatid separation, then ADCI needs more images to find those images that show strong cohesion between chromatids. ​
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 +=== Can I continue to use curves/​samples generated using an older version of ADCI after upgrading to a new version ===
 +It is important to note dicentric detection may vary across ADCI versions. Reasons for this variation include dicentric detection algorithm upgrades and software bug fixes. It is therefore recommended to analyse both calibration and test samples using the same version of ADCI. 
  
 ===== Useful tips and tricks ===== ===== Useful tips and tricks =====
main/faqandtips.txt · Last modified: 2018/06/26 19:26 by bshirley